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Other 관련 자주 묻는 질문과 그에 대한 답을 확인해보세요.

  • Cytiva에서 제공하는 Online Tool은 어떤 것이 있나요?
    실험에 유용한 많은 tool을 Web과 Mobile Apps으로 제공하고 있습니다.

    아래 링크에서 많은 정보를 얻으시길 바랍니다.

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  • 유용한 Mobile Apps을 소개해 주세요.

    Chromatography 를 위한 [Purify], [ÄKTA accessories], Lab filtration을 위한 [Whatman Filters], 그외에 [Percoll Calculator], [Oil Calculator] 등이 있습니다. Cytiva의 유용한 Mobile App들은 아래 페이지에서 확인하실 수 있습니다.

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  • Regulatory Support 문서는 어떻게 받을 수 있나요?
    Cytiva의 Regulatory Support Web Application Website에서 회원가입 하시면 원하시는 Regulatory Support File (RSF)을 직접 찾아서 pdf로 받으실 수 있을 뿐 아니라, regulatory support document (RSD)가 update 되거나, 새로운 Change Control Notification (CCN)이 publish 되면 이메일로 안내를 받으실 수 있습니다. 자세한 내용은 아래 '제품 문서 지원' 페이지에서 확인 바랍니다.

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  • Regulatory Support Web Application은 무엇인가요?
    GE Healthcare Life Sciences는 Regulated Environments에서 GE 제품을 활용할 수 있도록 regulatory support document (RSD)와 Change Control Notification (CCN)의 Regulatory Support를 Web상에서 고객에게 직접 제공합니다.

     
    Regulatory Support Document (RSD)
    Change Control Notification (CCN)
    소개규제기관에 제공할 수 있는 process development, validation, SOP, QC, 그리고 임상과 마케팅을 위한 자료들고객이 생산하는 제품, 공정, 문서, 또는 과정에 잠재적인 영향을 미칠 수 있는 변화에 대한 정보를 제공하는 서비스
    종류1) Regulatory Support Files (RSF) -> 제품의 기능, 안정성, extractable compounds, 분석방법 등을 수록한 자료1) BioProcess 제품 -> Chromatography resin/장비/columns, RTP, WAVE Bioreactors, Process-scale filtration
    2) Validation Support File (VSF) -> UNICORN software의 version에 따른 validation 자료2) Biacore 제품 -> Biacore 장비/software/소모품
    3) Validation Guide (VG) -> Single-use products (Process-scale filtration equipment, Cellbag bioreactors)과 ReadyToProcess products의 validation guide
  • Ficoll-Paque PREMIUM은 Ficoll-Paque PLUS와 어떤 차이가 있나요?
    Ficoll-Paque PREMIUM 세 가지 제품들은 30 년 전통의 Ficoll-Paque PLUS를 기본으로 해서 생산한 제품으로, chemical composition과 packaging에는 차이가 없습니다.
    차이점은 Ficoll-Paque PREMIUM 제품들은 의료 산업 분야의 품질 경영 인증 (QMS ; Quality Management System)인 ISO 13485:2003과 GMP 가이드라인에 따라서 엄격한 제조 공정으로 생산하였다는 점입니다.
    따라서, Ficoll-Paque PLUS은 연구용으로만 사용해야 하는 반면에 Ficoll-Paque PREMIUM은 연구용 외에도 In vitro Use가 가능합니다.

    (1) 비교 Table
    Ficoll-Paque PLUSFicoll-Paque PREMIUM
    UseFor Research Use OnlyFor In Vitro Use Only
    Quality ManufacturingNo QMSUnder Quality Management System certified to ISO 13485:2003
    RSF (Regulatory Support File)NoYes
    Max. Density① 1.078 g/ml (human blood; 17-1440-02 & 17-1440-03)① 1.078 g/ml (human blood; 17-5442-02 & 17-5442-03) ② 1.085 g/ml (rat & mice; 17-5446-02) ③ 1.073 g/ml (human bone marrow; 17-5446-52)
    ApplicationIsolation of lymphocytes from human peripheral bloodOptimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood
    Endotoxin Activity Max.< 0.12 EU/ml
    ColorColorless to slightly yellow
    Shelf life3 years
    pH range5.5 – 7.5
    Certificate of AnalysisYes
    (2) Web Link

    ① Ficoll-Paque PREMIUM : https://www.cytivalifesciences.com/en/kr/shop/cell-therapy/media/ficoll-paque-premium-density-gradient-media-p-05822

    ② Ficoll-Paque PLUS : https://www.cytivalifesciences.com/en/kr/shop/cell-therapy/media/ficoll-paque-plus-density-gradient-media-p-05824

    (3) Ordering information
    ProductPack sizeCode No.
    Ficoll-Paque PREMIUM6 × 100 ml17-5442-02
    Ficoll-Paque PREMIUM6 × 500 ml17-5442-03
    Ficoll-Paque PREMIUM 1.0846 × 100 ml17-5446-02
    Ficoll-Paque PREMIUM 1.0736 × 100 ml17-5446-52
    Ficoll-Paque PLUS6 × 100 ml17-1440-02
    Ficoll-Paque PLUS6 × 500 ml17-1440-03
  • TE70 Troubleshooting
    TE70은 Semi-dry transfer 장비이며, 전체 면적은 14 x 16cm입니다. Gel은 단층으로 2장을 side by side로 올릴 수 있고, Cellophane을 통해 stack을 한 개 더 올릴 수 있습니다.

     

    1. 위치 및 Stack (단층의 경우)

    소모품_FAQ_list_10

     

    2. Gel을 위로 한장 더 올릴 경우

    소모품_FAQ_list_11

     

    3. Power supply error

    ⓐ. Stack을 놓는 아랫판에 buffer의 양이 너무 많은 경우에 connection에 error가 생깁니다.

    ☞ Buffer는 stack의 가장 윗부분에 한번더 올려주는것으로 충분합니다.

    ⓑ. TE70의 Lead선과 power supply의 연결을 확인합니다.

     

    4. Transfer가 전혀 안됩니다!

    ☞ Transfer buffer의 pH확인할 것! 특히 CAPS buffer사용시에는 pH11을 확인하고, 일반 Towbin buffer시에는 pH보정 필요없습니다. (pH8.6)

     

    5. Transfer가 불안정합니다!

    ☞ Gel의 개수만큼 current계산을 확인할 것! 0.8mA/cm2 X Gel수를 체크하여 봅니다.
  • SE250 part 문의
    SE250은 10 x 8 cm의 mini gel 전기영동을 위한 장비입니다. 제품과 관련한 package등 part에 대한 정보는 아래와 같습니다. Gel Casters (Spare Parts)       
     80-6146-50, SE245 소모품_FAQ_list_1Dual gel caster, for casting 1-2 gels in 10 x 8 cm or 10 x 10.5 cm plates used in the SE 250, SE 260 or miniVE vertical electrophoresis unit. NOTE: Unit is shipped with the clamp screws facing inwards. When in use, the clamp assemblies should be aligned with the screws facing outwards. Includes: Casting cradle, 2 clamp assemblies, 2 gaskets and 4 black cams. (Order plates, spacers and combs separately.) Casting cradle, 2 clamp assemblies, 2 gaskets and 4 black cams. (Order plates, spacers and combs separately.) Casting cradle, 2 clamp assemblies, 2 gaskets and 4 black cams. (Order plates, spacers and combs separately.)
     80-6151-06, SE275* 소모품_FAQ_list_2Multiple Gel Caster (casts 2 to 4 gels, for SE250) 4-gel multiple gel caster (front), for casting 1-4 gels in 10 x 8 cm plates used in the SE 250 mini vertical electrophoresis unit. Includes: Casting chamber with foam rubber gasket, face plate, 10 rectangular glass plates (10 x 8 cm), 4 notched alumina plates (10 x 8 cm), 2 clamps, 100 sheets of wax paper, space-saver plate, 5 filler sheets, black vinyl cap and filler plug set. (Order SE 211A combs and SE 2119T spacers separately.)
     80-6142-51, SE215* 소모품_FAQ_list_3Multiple Gel Caster (casts 4 to 10 gels, for SE250) 10-gel multiple gel caster (shown on the right), for casting 4-10 gels in 10 x 8 cm plates used in the SE 250 mini vertical lectrophoresis unit. Includes: Casting chamber with foam rubber gasket, face plate, 20 rectangular glass plates (10 x 8 cm), 10 notched alumina plates (10 x 8 cm), 100 sheets of wax paper, 2 clamps, space saver plate, 5 filler sheets, black vinyl cap and filler plug set. (Order SE 211A combs and SE 2119T spacers separately.)
    GLASS PLATES The glass plates for the SE 250 and 260 are low fluorescing (they are water white drawn glass as opposed to float glass).
    Product Quantity Code number
     Glass plates, for SE250 (10 x 8 cm):
     Rectangular glass plates 10/pk 80-6136-81, SE202P-10*
     Notched alumina plates 10/pk 80-6136-43, SE202N-10*
     Notched glass plates 5/pk 80-6136-05, SE202GN-5*
     
     80-6148-40, SE254B* 소모품_FAQ_list_41 UBC. Includes foam gaskets.Upper buffer chamber (UBC cooling core), for SE250 and SE260. Replacement upper buffer chamber (UBC), for the SE 250 or SE 260 mini vertical electrophoresis unit. This UBC also serves as the cooling core.
     80-6137-19, SE208* 1Foam gasket, grey, 61 cm long x 4.5 mm diameter, for the UBC of the SE 250 or SE 260 mini vertical electrophoresis unit, or for the casting chamber of the SE 215, SE 275, SE 615 or SE 675 multiple gel caster.
     #26019* [Four of them are included in the SE250/260 Hardware Repair kit, 80-6291-66]Acetal rivets NOTE: These are the white pins that hold down the Pt wire in the UBC. They are also used in the Blot Module of the miniVE.
     80-6148-59, SE255* 소모품_FAQ_list_5 1Lower buffer chamber (LBC) Lower buffer chamber (LBC), for the SE 250 mini vertical electrophoresis unit.
     80-6148-78, SE255D* 소모품_FAQ_list_6 1For SE260 Deep lower buffer chamber (LBC), for the SE 260 mini vertical electrophoresis unit. This LBC can also convert an existing SE 250 unit into a SE 260, enabling the user to run a wider variety of precast gels.
     80-6149-16, SE256* 소모품_FAQ_list_7 1 lid assembly. Includes black and red high voltage leads.Lid ass'y with cables, Lid assembly with cables, for the SE 250 or SE 260 mini vertical electrophoresis unit.
     80-6177-09, SE6056-HV* 소모품_FAQ_list_8 1 red and 1 black high voltage leadsHigh voltage safety lead set; High voltage lead set, black and red. Female end is for use with Hoefer safety lids only. Male end is sheathed and may therefore require an adaptor to fit into certain 3rd-party power supplies.
     80-6147-83, SE252* 소모품_FAQ_list_9 4/pkSpring clamps, red, asymmetric  Replacement long red springclamps (4 per pack), current design, for the SE 250 or SE 260 mini vertical electrophoresis unit. These clamps have asymmetric claws, which exert a better clamping pressure on the exposed glass face. These clamps are also used to clamp the face plate to the caster body of the SE 215, SE 235, SE 275 and discontinued  SE 295 multiple gel casters. In the SE 245 dual gel caster, these clamps are used to hold the top edges of 10 x 10.5 cm or 10 x 12 cm glass plates that extend beyond the casting clamps.
     80-6148-02, SE253* 4/pkShort red clamps; Short red springclamps (4), old design, for the SE 250 or SE 260 mini vertical electrophoresis unit. These clamps are the original design, with symmetric claws so the exposed glass face doesn't get as much clamping as with the current asymmetric design (SE252). These clamps are also very tight when using spacers of 1.5 mm thickness or more.These clamps are also currently used in the SE 615 and SE 675 multiple gel casters
     80-6148-21, SE253W* 4/pkShort white clamps
     80-6142-13, SE212* 2/pkDecal, well-locating; Well-locating decals (2 per pack), for the SE 250 or SE 260 mini vertical electrophoresis unit, or for the discontinued Tall Mighty Small SE 280 mini vertical electrophoresis unit. These are small acetate sheets printed with teeth tracings of standard Hoefer combs. Placing these against the glass plate sandwich allows you to visualize the gel wells for easy sample loading. Reusable.
     80-6421-43 1/4 oz tubeGel seal
     Glass plates, combs and spacers are listed separately in
     80-6114-96, QF-1/4* 2/pkQuickFit connectors, female 1/4"
     80-6115-15, QFX-3/8* 2/pkQuickFit connectors, male 3/8"
     80-6291-66 1 kitRepair kit, SE250/260 Hardware. Includes: banana plugs (2), washers (6), nuts (2), PTFE tubing (three 3" lengths), acetal rivets (4)
    SPACERS
     Thickness (mm) Length (cm) Color QuantityCode number 
     0.75 8 grey 2/pk 80-6137-95, SE2119T-2-.75*
     1.00 8 white 2/pk 80-6138-14, SE2119T-2-1.0*
     1.50 8 grey 2/pk 80-6138-33, SE2119T-2-1.5*
     0.75 10.5 grey 2/pk 80-6149-92, SE2619T-2-.75*
     1.00 10.5 white 2/pk 80-6150-11, SE2619T-2-1.0*
     1.50 10.5 grey 2/pk 80-6150-30, SE2619T-2-1.5*
     0.75 12 grey 2/pk 80-6152-20, SE2819T-2-.75*
     1.00 12 ? 2/pk 80-6152-39, SE2819T-2-1.0*
     1.50 12 grey 2/pk 80-6152-58, SE2819T-2-1.5*
    COMBS Comb heights are 13 mm.   ;
     Number of wells Comb thickness (mm) Well width (mm) Well volume (µl per 1 mm depth) Quantity Code number
    5 0.75 13.0 9.8 1 80-6140-23, SE211A-5-.75*
    5 1.00 13.0 13.0 1 80-6140-42, SE211A-5-1.0*
    5 1.50 13.0 19.5 1 80-6140-61, SE211A-5-1.5*
    9† 1.00 5.8 5.8 1 1.00 5.8 5.8 1 80-6140-80, SE211A-9-1.0*
     10 0.75 4.8 3.6 1 80-6138-71, SE211A-10-.75*
     10 1.00 4.8 4.8 1 80-6138-90, SE211A-10-1.0*
     10 1.50 4.8 7.2 1 80-6139-09, SE211A-10-1.5*
     15 0.75 2.9 2.2 1 80-6139-47, SE211A-15-.75*
     15 1.00 2.9 2.9 1 80-6139-66, SE211A-15-1.0*
     15 1.50 2.9 4.4 1 80-6139-85, SE211A-15-1.5*
     18† 1.00 2.9 2.9 1 1.00 2.9 2.9 1 80-6140-04, SE211A-18-1.0*
     blank blank blank blank 1 80-6140-99, SE211A-B-.75*
     blank blank blank blank 1 80-6141-18, SE211A-B-1.0*
     blank blank blank blank 1 80-6141-37, SE211A-B-1.5*
     prep 1/1†† 0.75 68/5 51/3.8 1 0.75 68/5 51/3.8 1 80-6141-56, SE211A-R-.75*
     prep 1/1†† 1.00 68/5 68/5 1 1.00 68/5 68/5 1 80-6141-75, SE211A-R-1.0*
     prep 1/1†† 1.50 68/5 102/7.5 1 1.50 68/5 102/7.5 1 80-6141-94, SE211A-R-1.5*
    *Old Hoefer part designation †microtiter spacing ††preparative/reference well

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